8-base-pair i7 nextera barcode  (Nextera AS)

Journal: ACS Omega

Article Title: uPIC–M: Efficient and Scalable Preparation of Clonal Single Mutant Libraries for High-Throughput Protein Biochemistry

doi: 10.1021/acsomega.1c04180

Figure Lengend Snippet: Overview of the uPIC–M pipeline to generate user-defined clonal mutant libraries. (A) Examples of clonal libraries from uPIC–M and potential high-throughput biochemistry applications. Applications are listed along with examples of the types of variants involved. (B) Comparison of cost (including materials and labor) of conventional mutagenesis vs uPIC–M for libraries of 50–20,000 mutants. A uPIC–M clone sampling rate of 384 per 50 desired mutants (7.68-fold excess) was used for these calculations. uPIC–M (modified) represents a lower cost version of uPIC–M with the addition of pipet tip washing for plate liquid transfer steps. See Table S1 for full time and cost calculations. (C) Workflow for generating uPIC–M libraries in three phases: (1) Mutagenic oligos are synthesized for ∼50 residue windows on a pooled array and selective PCR amplification of each window generates a primer pool used for QuikChange; (2) pooled QuikChange reactions are transformed and plated, with each plate containing a mixture of ∼50 possible single mutants, facilitating colony picking into multiwell plates to isolate clonal libraries of unidentified variants; (3) clonal libraries are prepared and sequenced by NGS to reveal the genotype and location of each variant.

Article Snippet: We used portions of an available 7680 member (20 × 384) dual unique indexed i5/i7 Nextera barcode library.

Techniques: Mutagenesis, High Throughput Screening Assay, Comparison, Sampling, Modification, Synthesized, Residue, Amplification, Transformation Assay, Variant Assay

Journal: Molecular cell

Article Title: A high-throughput screen for transcription activation domains reveals their sequence characteristics and permits reliable prediction by deep learning

doi: 10.1016/j.molcel.2020.04.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: nextera i7 barcodes , Illumina , UDP0001-UDP0096.

Techniques: Recombinant, DNA Sequencing, Sequencing, Plasmid Preparation, Software, SYBR Green Assay